Subaiba Shafi Kadakkadan
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← Research Cavendish Living Lab, University of Westminster

Bacterial cellulose and biopolymers from food waste

May – Oct 2023 Biopolymer Scientist Intern, bioplastics strand

Producing bacterial cellulose from food-waste substrates with Komagataeibacter xylinus, as part of a student-led sustainability lab on the Westminster campus.

BiopolymersBacterial celluloseFermentationSustainability

Cavendish Living Lab website ↗

The Living Lab

The Cavendish Living Lab is a vertically integrated project at the University of Westminster. Students from different disciplines and years use the campus as a laboratory to co-create solutions to food waste, plastic waste and wastewater. It is led by Dr Pooja Basnett, with Dr Dipankar Sengupta and Dr Linda Percy, and funded by the Quintin Hogg Trust. Its three strands are urban food growing, bioplastics and water management. The project was a finalist in the 2023 Green Gown Awards (Tomorrow’s Employees).

My work

I worked in the bioplastics strand, where the aim was to make sustainable materials from the university’s own waste streams.

  • Screened food and drink waste as carbon sources for bacterial cellulose, in 24-well plates and then in flasks of Hestrin–Schramm medium: fruit and vegetable waste, juices, ciders, coffee waste, beetroot, turmeric and spinach, among others.
  • Produced bacterial cellulose from food-waste substrates using Komagataeibacter xylinus, increasing yield by 5%.
  • With the team, tested how processing changes the material: bleaching; natural dyes (turmeric, spinach, coffee, indigo, beetroot, raspberry); air-drying versus freeze-drying; blending with kombucha cellulose, soybean, zein and seaweed; and growing cellulose in 3D-printed moulds.
  • Prototyped bacterial cellulose as a wearable material, and exhibited the materials at the Cavendish Living Lab Symposium 2023.
  • Contributed to work on polyhydroxyalkanoates (PHAs), another family of microbially produced bioplastics.
  • Contributed to cross-disciplinary projects linking hydroponic food growing with microbial biofilm production.
  • Co-led project documentation and presented results to internal research committees.

What I took from it

Fermentation is where microbiology meets process constraints: substrate composition, yield and reproducibility decide whether a product is useful. That way of thinking carries straight into natural-product work, where culture conditions decide what a microbe makes.

Photos

The 2023 Biopolymer Scientist team, as announced by the Cavendish Living LabCavendish Living Lab
The team's bacterial cellulose display: dyes, drying methods, blended substrates, 3D-printed moulds and wearable prototypesCavendish Living Lab
Bacterial cellulose tested as a wearable material on miniature dress formsCavendish Living Lab
Growing a bacterial cellulose mat in static cultureCavendish Living Lab
Dried bacterial cellulose samples from different substrates and treatmentsCavendish Living Lab
Our bacterial cellulose stall at the Cavendish Living Lab Symposium 2023: pellicles, bleached and dyed cellulose, and a 3D-printed mouldCavendish Living Lab
The Cavendish Living Lab talk series, 2023Cavendish Living Lab
A talk on food waste and urban food growing at a Living Lab eventCavendish Living Lab
Lifting a bacterial cellulose pellicle from a culture bottleCavendish Living Lab
Hestrin–Schramm medium supplemented with turmeric, spinach, beetroot and coffee waste, July 2023Cavendish Living Lab
Screening food-waste substrates in a 24-well plate, June 2023Cavendish Living Lab
A second substrate screen, including cola, coffee waste and juice residues, June 2023Cavendish Living Lab
Starter cultures in Hestrin–Schramm medium, July 2023Cavendish Living Lab
The team's plan for the Biopolymer Scientist internshipCavendish Living Lab